| Human SIRP alpha/CD172a Protein (LTP10747) |
| LTP10747 |
| 100ug |
|
$406 In stock |
| Signal regulatory protein ? (SIRP?) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRP? acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "donĀ“t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP ? and inhibit macrophage-mediated destruction. |
| Recombinant Human SIRP alpha/CD172a Protein is expressed from Expi293 with His tag at the C-terminal. It contains Glu31-Arg370. |
| SIRP alpha/CD172a |
| Human |
| P78324-1 |
| Glu31-Arg370 |
| The protein has a predicted MW of 38.1 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on the Tris-Bis PAGE result. |
| Immobilized Human SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 99.1ng/ml determined by ELISA (QC Test). The affinity constant of 20.6nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| C-His |
| Expi293 |
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| ![Human SIRP alpha/CD172a Protein (LTP10747)](/peptide-product/images/protein-products/LTP10747/20211210133136.png) |