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| Human Tim-3/HAVCR2 Protein (LTP10745) |
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| LTP10745 |
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| 100ug |
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$360 In stock |
| Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 gene.TIM3 is an immune checkpoint and together with other inhibitory receptors including programmed cell death protein 1 (PD-1) and lymphocyte activation gene 3 protein (LAG3) mediate the CD8 T-cell exhaustion. TIM3 has also been shown as a CD4 Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. |
| Recombinant Human Tim-3/HAVCR2 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Ser22-Arg200. |
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| Tim-3/HAVCR2 |
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| Human |
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| Q8TDQ0-1 |
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| Ser22-Arg200 |
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| The protein has a predicted MW of 20.7 kDa, Due to glycosylation, the protein migrates to 50-60 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human TIM-3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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