|
| Human TNFR1/CD120a/TNFRSF1A Protein (LTP10632) |
|
| LTP10632 |
|
| 100ug |
|
|
$327 In stock |
| Tumour necrosis factor alpha (TNF-?) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-?, while sparing or potentiating the protective effects of TNFR2 signalling. |
| Recombinant Human TNFR1/CD120a/TNFRSF1A Protein is expressed from Expi293 with His tag at the C-terminal. It contains Leu30-Thr211. |
|
| TNFR1/CD120a/TNFRSF1A |
|
| Human |
|
| P19438-1 |
|
| Leu30-Thr211 |
|
| The protein has a predicted MW of 23.3 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on the Tris-Bis PAGE result. |
|
| Immobilized Human TNFR1, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 4.8ng/ml determined by ELISA. See testing image for detail. |
|
| C-His |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
