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| Human Transferrin R/CD71 Protein (LTP11034) |
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| LTP11034 |
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| 100ug |
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$406 In stock |
| The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status. |
| Recombinant Human Transferrin R/CD71 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Cys89-Phe760. |
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| Transferrin R/CD71 |
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| Human |
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| P02786 |
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| Cys89-Phe760 |
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| The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-80 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human Transferrin R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA. The affinity constant of 27.50 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| N-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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