Human TSLP Protein (LTP10843)

Product Name
Human TSLP Protein (LTP10843)
Catalog Number
LTP10843
Quantity
100ug
Price
$473 In stock
Background
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
Description
Recombinant Human TSLP Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Tyr29-Gln159.
Target
TSLP
Species
Human
Gene/Protein ID
Q969D9-1
Exact Sequence
Tyr29-Gln159
Molecular Weight
The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 12 kDa&20-25 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human TSLP, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 0.1ug/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human TSLP Protein (LTP10843)
  • 5 Units in Stock

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