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| Human VEGF121 Protein (LTP10502) |
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| LTP10502 |
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| 100ug |
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$519 In stock |
| Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. |
| Recombinant Human VEGF121 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ala27-Arg147. |
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| VEGF121 |
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| Human |
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| P15692-9 |
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| Ala27-Arg147 |
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| The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reducing (N) condition based on the Tris-Bis PAGE result. |
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| Immobilized Human VEGF121, His Tag at 2ug/ml (100ul/Well). Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 42ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS,250mM Arginine (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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