Human VEGF165 Protein (LTP10849)

Product Name
Human VEGF165 Protein (LTP10849)
Catalog Number
LTP10849
Quantity
100ug
Price
$450 In stock
Background
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
Description
Recombinant Human VEGF165 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ala27-Arg191.
Target
VEGF165
Species
Human
Gene/Protein ID
P15692-4
Exact Sequence
Ala27-Arg191
Molecular Weight
The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reducing (N) condition based on the Tris-Bis PAGE result.
Activity
Immobilized Human VEGF165 at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human VEGF165 Protein (LTP10849)
  • 5 Units in Stock

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