| Human VEGF165 Protein (LTP10849) |
| LTP10849 |
| 100ug |
|
$450 In stock |
| Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. |
| Recombinant Human VEGF165 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ala27-Arg191. |
| VEGF165 |
| Human |
| P15692-4 |
| Ala27-Arg191 |
| The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reducing (N) condition based on the Tris-Bis PAGE result. |
| Immobilized Human VEGF165 at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA. See testing image for detail. |
| C-His-Avi |
| Expi293 |
| > 95% as determined by Tris-Bis PAGE |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| ![Human VEGF165 Protein (LTP10849)](/peptide-product/images/protein-products/LTP10849/20230206094949.png) |