Human VEGF-C/Flt4-L Protein (LTP10610)

Product Name
Human VEGF-C/Flt4-L Protein (LTP10610)
Catalog Number
LTP10610
Quantity
100ug
Price
$383 In stock
Background
the lymphangiogenic factors vascular endothelial growth factor C (VEGFC) and VEGFD are cleaved by thrombin and plasmin, serine proteases generated during hemostasis and wound healing. Genetic studies reveal that platelet enhancement of lymphatic growth after wounding is dependent on the release of VEGFC, but not VEGFD, a finding consistent with high expression of VEGFC in both platelets and avian thrombocytes.
Description
Recombinant Human VEGF-C/Flt4-L Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Thr103-Arg227.
Target
VEGF-C/Flt4-L
Species
Human
Gene/Protein ID
Q6FH59
Exact Sequence
Thr103-Arg227
Molecular Weight
The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.29 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCI (pH 6.0).

Image
Human VEGF-C/Flt4-L Protein (LTP10610)
  • 5 Units in Stock

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