Human WT-1 (HLA-A*02:01) Protein (LTP10793)

Product Name
Human WT-1 (HLA-A*02:01) Protein (LTP10793)
Catalog Number
LTP10793
Quantity
100ug
Price
$406 In stock
Background
The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and RMFPNAPYL peptide of  the WT-1.
Description
Recombinant Human WT-1 (HLA-A*02:01) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide.
Target
WT-1 (HLA-A*02:01)
Species
Human
Gene/Protein ID
A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL
Exact Sequence
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and RMFPNAPYL peptide
Molecular Weight
The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human WT-1 (HLA-A*02:01) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-WT-1 Antibody, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.33nM as determined in SPR assay (Biacore T200). See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human WT-1 (HLA-A*02:01) Protein (LTP10793)
  • 5 Units in Stock

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