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| Human WT-1 (HLA-A*02:01) Protein (LTP10793) |
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| LTP10793 |
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| 100ug |
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$406 In stock |
| The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and RMFPNAPYL peptide of the WT-1. |
| Recombinant Human WT-1 (HLA-A*02:01) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide. |
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| WT-1 (HLA-A*02:01) |
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| Human |
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| A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL |
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| Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and RMFPNAPYL peptide |
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| The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human WT-1 (HLA-A*02:01) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-WT-1 Antibody, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.33nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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