Human/Cynomolgus/Rhesus macaque CD28 Protein (LTP10785)

Product Name
Human/Cynomolgus/Rhesus macaque CD28 Protein (LTP10785)
Catalog Number
LTP10785
Quantity
100ug
Price
$450 In stock
Background
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Description
Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Asn19-Pro152.
Target
CD28
Species
Human/Cynomolgus/Rhesus macaque
Gene/Protein ID
P10747-1
Exact Sequence
Asn19-Pro152
Molecular Weight
The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA (QC Test). The affinity constant of 5.15nM as determined in SPR assay (Biacore T200). See testing image for detail.
Tag
N-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human/Cynomolgus/Rhesus macaque CD28 Protein (LTP10785)
  • 5 Units in Stock

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