| The Internal Transcribed Spacers (ITS) rRNA blocker with the sequence CGAGGGCACGTCTGCCTGG is designed to target and block specific regions within the internal transcribed spacer (ITS) of rRNA genes. The ITS regions are located between conserved rRNA genes such as 18S, 5.8S, and 28S, playing a crucial role in the processing and maturation of rRNA during ribosome biogenesis. The Reverse Complementary Sequence (5' to 3') is CCAGGCAGACGTGCCCTCG.
Function:
The ITS regions are non-coding sequences that are transcribed along with rRNA genes but are cleaved during the maturation process to form functional rRNA. Blocking ITS sequences can interfere with the proper maturation and assembly of rRNA, affecting ribosomal function. By blocking these regions, researchers can inhibit ribosome formation, which can lead to reduced protein synthesis.
Applications in Biological Research:
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Phylogenetic Studies: ITS regions are highly variable and are widely used as molecular markers in phylogenetic and taxonomic studies, particularly in fungi, plants, and some animals. The ITS blocker can be used to study the functional importance of ITS regions by preventing their maturation during rRNA processing, providing insights into their evolutionary conservation and role in ribosome assembly.
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Fungal and Microbial Taxonomy: ITS regions serve as barcodes for identifying species in microbial and fungal taxonomy. The use of ITS blockers in these studies can help in differentiating between closely related species and understanding the role of ITS variability in species identification.
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Ribosomal Function and Genetic Studies: Inhibiting ITS processing with this blocker allows researchers to investigate the functional roles of different parts of the rRNA gene cluster in ribosome biogenesis, gene expression regulation, and cellular processes dependent on efficient protein synthesis.
Overall, this ITS blocker is a valuable tool for exploring the structural and functional roles of internal transcribed spacers in rRNA maturation and for studying the broader impacts on ribosomal function and species evolution. |
