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| [KO Validated] HMGB1 Rabbit mAb |
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| LTA24699 |
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| 100ul |
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$500
In stock
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| HMGB1 Rabbit mAb is produced by immunizing Rabbit with a peptide of human HMGB1. |
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| HMG-1;HMG1;HMG3;SBP-1;HMGB1 |
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| Human |
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| 3146 |
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| P09429 |
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| A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (P09429). Peptide sequence: SAFFLFCSEY RPKIKGEHPG LSIGDVAKKL GEMWNNTAAD DKQPYEKKAA KLKEKYEKDI AAYRAKGKPD AAKKGVVKAE KSKKKKEEEE DEEDEEDEEE E |
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| Rabbit |
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| Monoclonal |
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| WB,IHC,IF/ICC |
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| Human,Mouse,Rat |
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| 25kDa |
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| WB,1:500 - 1:2000|IHC-P,1:50 - 1:100|IF/ICC,1:50 - 1:100 |
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| Store at -20?. Avoid freeze/thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
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Shipped at ambient temperature.
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Centrifuge tubes before opening. Dissolve the lyophilized antibodies in distilled water. 5-10% glycerol is recommended.
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Western blot analysis of extracts of various cell lines, using HMGB1 antibody (LTA24699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
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