|
| LAMC1 Rabbit mAb |
|
| LTA24813 |
|
| 100ul |
|
|
$750
In stock
|
|
| Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. |
|
| LAMB2 |
|
| 3915,LAMC1,LAMB2,laminin subunit gamma 1,laminin subunit gamma-1,S-LAM gamma,S-laminin subunit gamma,laminin B2 chain,laminin,gamma 1 (formerly LAMB2),laminin-10 subunit gamma,laminin-11 subunit gamma,laminin-2 subunit gamma,laminin-3 subunit gamma,laminin-4 subunit gamma,laminin-6 subunit gamma,laminin-7 subunit gamma,laminin-8 subunit gamma,laminin-9 subunit gamma,P11047,Laminin Subunit Gamma 1,Laminin,Gamma 1 (Formerly LAMB2),Laminin-10 Subunit Gamma,Laminin-11 Subunit Gamma,Laminin-2 Subunit Gamma,Laminin-3 Subunit Gamma,Laminin-4 Subunit Gamma,Laminin-6 Subunit Gamma,Laminin-7 Subunit Gamma,Laminin-8 Subunit Gamma,Laminin-9 Subunit Gamma,S-Laminin Subunit Gamma,Laminin B2 Chain,S-LAM Gamma,Laminin Subunit Gamma-1,Laminin-1 Subunit Gamma |
|
| Human |
|
| 3915 |
|
| P11047 |
|
| ECPACYRLVKDKVADHRVKLQELESLIANLGTGDEMVTDQAFEDRLKEAEREVMDLLREAQDVKDVDQNLMDRLQRVNNTLSSQISRLQNIRNTIEETGNLAEQARAHVENTERLIEIASRELEKAKVAAANVSVTQPESTGDPNNMTLLAEEARKLAERHKQEADDIVRVAKTANDTSTEAYNLLLRTLAGENQTAFEIEELNRKYEQAKNISQDLEKQAARVHEEAKRAGDKAVEIYASVAQLS |
|
| Recombinant fusion protein containing a sequence corresponding to amino acids 1030-1275 of human LAMC1 (NP_002284.3). |
|
| Rabbit |
|
| Affinity Purified Monoclonal IgG |
|
| WB, ELISA |
|
| Mouse, Rat |
|
| 178kDa |
|
| WB,1:1500 - 1:3000|ELISA,Recommended starting concentration is 1 _g/mL. Please optimize the concentration based on your specific assay requirements. |
|
|
Reconstituted antibodies stable at -80C for 12 months, 4C for 1 week. Avoid repeated freeze-thaw cycles.
|
|
|
Shipped at ambient temperature.
|
|
|
Centrifuge tubes before opening. Dissolve the lyophilized antibodies in distilled water. 5-10% glycerol is recommended.
|
|
| .jpg )
Western blot analysis of various lysates using LAMC1 Rabbit mAb (LTA24813) at 1:3000 dilution incubated overnight at 4_.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LT014) at 1:10000 dilution.|Lysates/proteins: 25 _g per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (LT00020).|Exposure time: 90s. |
