| The Mitochondrial rRNA blocker with the sequence GGCAAGTGTTCTTCGGA is designed to target mitochondrial ribosomal RNA (rRNA), specifically blocking the function of 12S or 16S mitochondrial rRNAs. These rRNAs are essential components of the mitochondrial ribosome, which is responsible for translating the 13 key proteins involved in the oxidative phosphorylation pathway, a critical process for cellular energy production. Teh Reverse Complementary Sequence (5' to 3') is TCCGAAGAACACTTGCC.
Function:
This rRNA blocker works by binding to a complementary sequence within the mitochondrial rRNA, potentially inhibiting the function of the mitochondrial ribosome. This interference can block the translation of mitochondrial-encoded proteins, which could result in impaired mitochondrial function.
Applications:
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Mitochondrial Dysfunction Studies: Researchers use such blockers to study the role of mitochondria in various diseases, particularly those related to energy production disorders, such as mitochondrial myopathies or neurodegenerative diseases like Parkinson's and Alzheimer's.
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Cancer Research: Mitochondrial rRNA modification and translation are key processes that can be manipulated in cancer research, especially in understanding how mitochondria contribute to the metabolism and survival of cancer cells. By using blockers to inhibit mitochondrial rRNA, researchers can explore the effects of impaired mitochondrial function on cancer cell growth and treatment responses.
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Phylogenetic Studies: Mitochondrial rRNA genes, such as 12S and 16S, are also used as molecular markers for studying evolutionary relationships between species. Blockers targeting these rRNAs can help in experimental setups aimed at understanding the role of these genes in evolution.
This tool is crucial for mitochondrial research, providing insights into the regulatory mechanisms of mitochondrial gene expression and the broader implications of mitochondrial function in health and disease. |
