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| Mouse CD73/NT5E Protein (LTP10802) |
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| LTP10802 |
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| 100ug |
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$406 In stock |
| CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside?5'?monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. |
| Recombinant Mouse CD73/NT5E Protein is expressed from Expi293 with His tag at the C-terminal. It contains Trp29-Lys549. |
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| CD73/NT5E |
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| Mouse |
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| Q61503 |
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| Trp29-Lys549 |
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| The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Mouse CD73, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA (QC Test). See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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