|
LT441777 |
|
1 ml prepacked column |
|
6% highly cross-linked spherical agarose |
|
Ni Sepharose Fast Flow |
|
~40 mg histidine-tagged protein |
| 45~165 μm |
| 300 cm/h |
|
~15 µmol Ni2₊/ml medium |
|
pH 2-14 |
| 4 °C to 8 °C in 20% ethanol |
| Ni Sepharose Fast Flow is used for capture and intermediate purification. Ni Sepharose Fast Flow has a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2₊ ions for purification of histidine tagged proteins. Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea
|
|
- Use the prepacked column for easier process.
- The target molecule should be stable under the selected elution conditions.
- Avoid air bubbles in the column.
- It is recommended to regenerate the resin by 4 column volumes of 0.1 M glycine-HCl (pH 2.7) after 5 separation cycles.
- After 10 separation cycles, wash the resin with 5 column volumes of 20% ethanol and 5~10 column volumes of 70% ethanol sequentially to remove hydrophobic substances.
- For longer periods of storage, keep at 4~8ºC in 20% ethanol.
|
| For research purposes only. Not for human or animal therapeutic or diagnostic use. |
