Recharging Ni-NTA Resin Ni-NTA resin can be recharged following the procedures below: - For 1 ml of resin, wash with 2 ml of 7 M GuHCl. - Wash resin with 5 ml of deionized water. - Wash resin with 3 ml of 2% SDS. - Wash resin with 5 ml of deionized water. - Wash resin with 5 ml of 100% EtOH - Wash resin with 5 ml of deionized water. - Wash resin with 5 ml of 100 mM EDTA (pH 8) to strip Ni+2 ions. - Wash resin with 5 ml of deionized water. - Wash resin with 5 ml of 100 mM NiSO4. - Wash resin with 10 ml of deionized water. - Preserve resin in 0.02% azide or 20% ethanol. 
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