Faster Magnetic Response; Simpler Protocols;
LifeTein provides a series of magnetic beads for any bioseparation application, from microliter scale to liter scale. Our Magnetic Beads are nano-superparamagnetic beads covalently coated with highly functional groups or compounds such as maleimide, primary amine, NHS (N-hydroxysuccinimide), carboxylic acid, purified streptavidin, protein A, reduced glutathione, nickel-charged nitrilotriacetic acid, and groups for DNA/RNA purification.
|
Ni-NTA (His-tag Affinity) Magnetic Beads |
|
LTM005 |
|
20mg in 20% Ethanol
The magnet is not provided. |
|
10-60ug His-Tagged fusion proteins per mg magnetic beads |
|
500nm diameter |
|
Product shipped at room temp. Upon receipt, please store at 2-8°C.
|
| Stable for at least two years |
|
Ni-NTA Magnetic Beads are nano-superparamagnetic beads coupled with nickel-charged nitrilotriacetic acid (Ni-NTA). With a fast magnetic response rate, high protein binding capacity and low non-specific binding, the Ni-NTA Magnetic Beads provide a rapid and efficient method to purify His-tagged fusion proteins from crude cell lysates.
|
|
NTA has four chelation sites for nickel ions and thus binds nickel more tightly than other metal-chelating purification systems that only have three sites available for interaction with metal ions. The extra chelation site prevents nickel ion leaching and results in a greater binding capacity and higher purity of the extracted protein than those obtained using other metal-chelating purification systems. Can be re-used after bead regeneration. Binding capacity: 10-60 µg His-tagged fusion proteins per mg beads.
|
|
The beads are simply added to a sample and His-tagged fusion proteins will bind to the beads.
After washing unbound impurity off, the proteins can be eluted from the magnetic beads or the bound magnetic beads can be directly used in downstream experiments.
The process can be completed manually or fully automated for high throughput applications. |
|
The product is not thoroughly tested and is not intended for human use. For
in-vitro and research use only.
|
