| The ovalbumin peptide DEVSGLEQLESIINFEKL is a modified peptide derived from the well-studied SIINFEKL sequence, which is known for its role in antigen presentation through the MHC class I pathway. This peptide has critical applications in immunological research, particularly for studying antigen-specific T cell responses.
Functions:
This peptide sequence can act as a model antigen, mimicking the process by which T cells recognize infected or cancerous cells. The SIINFEKL portion binds to H-2Kb MHC class I molecules and presents the antigen on the surface of cells, which activates CD8+ T cells. This makes it a crucial tool for research in cell-mediated immunity, particularly in understanding how the immune system detects and responds to foreign pathogens or tumor cells.
Applications in Research:
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Immunological Studies: The peptide is widely used to investigate T-cell receptor (TCR) recognition and activation in both in vitro and in vivo systems. It helps researchers track antigen-specific CD8+ T cells and study their behavior during immune responses.
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Cancer Research: Due to its role in activating cytotoxic T cells, the peptide has been employed in tumor immunology to develop cancer immunotherapies, such as cancer vaccines, by stimulating the body’s immune system to target tumor cells displaying the antigen.
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Vaccine Development: It serves as a key component in experimental vaccines, especially in studying how to design vaccines that effectively induce strong CD8+ T-cell responses. This peptide is used to evaluate the efficacy of vaccine formulations and delivery methods that aim to improve immune responses against pathogens like viruses and bacteria.
Role in Drug Studies:
In drug development, this peptide is utilized to evaluate potential immunotherapies and vaccine candidates. It enables the testing of how different drug compounds affect antigen processing and presentation, and the subsequent immune activation. By tracking T cell responses to the peptide, researchers can determine the immunogenicity of different formulations and compounds, aiding the design of more effective therapies for diseases such as cancer and autoimmune disorders.
The DEVSGLEQLESIINFEKL peptide variant is particularly useful in experimental setups that aim to study not only the immune response but also the structural and functional aspects of antigen recognition and presentation by MHC molecules
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| | 1. Aurora A controls CD8+ T cell cytotoxic activity and antiviral response; Scientific Reports volume 9, Article number: 2211 (2019);
https://www.nature.com/articles/s41598-019-38647-y
...Ovoalbumin peptide (SIINFEKL) was synthesized by LifeTein...
2. Jeon D, Hill E, Moseman JE, McNeel DG. Combining toll-like receptor agonists with immune checkpoint blockade affects antitumor vaccine efficacy. J Immunother Cancer. 2024 May 3;12(5):e008799. doi: 10.1136/jitc-2024-008799. PMID: 38702146; PMCID: PMC11086196.
...Ovalbumin peptides for H-2b-restricted class I epitope (SIINFEKL) and class II epitope (ISQAVHAAHAEINEAGR, OVA-II) were synthesized, and the purity and identity were confirmed by mass spectrometry and gas chromatography (LifeTein, Hillsborough, New Jersey, USA)....
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