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LifeTein synthesized a molecule containing both the influenza neuraminidase (NA) inhibitor phosphate-oseltamivir and d-biotin, connected via an undecaethylene glycol spacer. The phosphor-oseltamivir–biotin conjugate inhibits influenza virus neuraminidase. The compound is very stable on streptavidin-coated biosensors. The compound is a potential candidate for the selective immobilization of the influenza virus in diagnosis, vaccine choice, development, or testing.
The Phospha Oseltamivir Biotin Conjugate is a specialized molecular tool designed for research and drug development, particularly targeting the influenza virus. This conjugate combines phosphate-oseltamivir, a neuraminidase inhibitor analog of oseltamivir (Tamiflu), with biotin, allowing it to probe biological systems. Using biotin, the conjugate can interact with common detection systems like avidin or streptavidin, facilitating binding and visualization in research assays.
Functions
- Influenza Neuraminidase Inhibition: Phospha oseltamivir retains strong inhibitory action against neuraminidase, the key enzyme influenza viruses use to replicate and spread. This inhibition is critical for halting viral activity, which makes it a powerful antiviral candidate.
- Probe for Virus Detection: Coupling with biotin enables the conjugate to act as a marker for the influenza virus, allowing researchers to detect viral presence and quantify viral neuraminidase activity in infected biological samples.
Applications
- Influenza Research: This conjugate is used to study the mechanism of neuraminidase inhibitors and their impact on viral replication. By binding specifically to neuraminidase, researchers can assess how effective such inhibitors are in preventing viral spread, making it a valuable tool in studying antiviral resistance.
- Drug Development: The conjugate helps screen potential antiviral compounds by providing a model for the behavior of neuraminidase inhibitors like oseltamivir. The biotin conjugate simplifies visualization in assays, supporting drug discovery efforts.
- Fluorescent or Clickable Probes: Beyond simple detection, biotin conjugates can be modified with other markers, such as fluorescent tags, enhancing their utility in imaging studies of viral infection and drug efficacy.
Usage in Scientific Research
Phospha oseltamivir conjugates, like this biotin derivative, are frequently used in biochemical assays to study the interaction between neuraminidase and its inhibitors. Researchers can use these conjugates in high-throughput screening assays to assess the strength and specificity of neuraminidase binding, aiding in developing new therapeutic strategies against influenza?.
This conjugate also plays a role in understanding resistance mechanisms in influenza strains, as the ability to detect and quantify neuraminidase activity informs researchers about potential drug-resistant viral mutations. |
