PMEL Rabbit mAb

Product Name
PMEL Rabbit mAb
Catalog Number
LTA24755
Quantity
100ul
Price
$750 In stock
Description
This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.
Alias
P1; SI; SIL; ME20; P100; SILV; HMB45; ME20M; gp100; HMB-45; ME20-M; PMEL17; D12S53E
Keywords
6490,PMEL,D12S53E,ME20,ME20-M,ME20M,P1,P100,PMEL17,SI,SIL,SILV,gp100,premelanosome protein,melanocyte protein PMEL,melanocyte protein Pmel 17,melanocyte protein mel 17,melanocytes lineage-specific antigen GP100,melanoma-associated ME20 antigen,melanosomal matrix protein17,silver locus protein homolog,silver,mouse,homolog of,P40967,Premelanosome Protein,Melanocytes Lineage-Specific Antigen GP100,Melanoma-Associated ME20 Antigen,Silver Locus Protein Homolog,Melanocyte Protein Pmel 17,Melanosomal Matrix Protein17,Silver (Mouse Homolog) Like,Melanocyte Protein Mel 17,Silver,Mouse,Homolog Of,Melanocyte Protein PMEL,Silver Homolog (Mouse),Gp100
Gene Template Species
Human
Gene/Protein ID
6490
SWISS ID
P40967
Antigen Sequence
KVPRNQDWLG VSRQLRTKAW NRQLYPEWTE AQRLDCWRGG QVSLKVSNDG PTLIGANASF SIALNFPGSQ KVLPDGQVIW VNNTIINGSQ VWGGQPVYPQ ETDDACIFPD GGPCPSGSWS QKRSFVYVWK TWGQYWQVLG GPVSGLSIGT GRAMLGTHTM EVTVYHRRGS RSYVPLAHSS SAFTITD
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-211 of human PMEL (NP_008859.1).
Animal Source
Rabbit
Antibody Type
Affinity Purified Monoclonal IgG
Applications
WB, ELISA
Cross-Reactivity
Human
Molecular Weight
70kDa
Recommended Dilution
WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 _g/mL. Please optimize the concentration based on your specific assay requirements.
Stability and Storage

Reconstituted antibodies stable at -80C for 12 months, 4C for 1 week. Avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Dissolve the lyophilized antibodies in distilled water. 5-10% glycerol is recommended.

Image
 PMEL Rabbit mAb

Western blot analysis of various lysates using PMEL Rabbit mAb (LTA24755) at 1:1000 dilution incubated overnight at 4_.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LT014) at 1:10000 dilution.|Lysates/proteins: 25 _g per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (LT00020).|Negative control (NC): MOLT-4|Exposure time: 90s.

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