| The PNA oligo Lys-TAACGGTATTTGGAG-Lys is a synthetic peptide nucleic acid (PNA) designed to target human globin mRNA. It binds specifically to globin mRNA sequences, thus blocking their amplification during reverse transcription and PCR processes. This is particularly useful in RNA sequencing applications, as globin mRNA, which makes up a significant portion of total mRNA in blood samples, can interfere with detecting and analyzing non-globin mRNA. By preventing globin mRNA from being amplified, the PNA oligo allows researchers to focus on other mRNA transcripts, improving the sensitivity and accuracy of whole-blood transcriptome analysis. Other sequences are Lys-GTAGTTGGACTTAGG-Lys, Lys-ATCCAGATGCTCAAG-Lys, and Lys-GCCCTTCATAATATC-Lys.
Applications in Biological Research:
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RNA Sequencing: The PNA oligos are used to reduce the impact of globin mRNA in blood-derived RNA samples, ensuring that non-globin transcripts are more readily detected and amplified.
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Gene Expression Studies: In studies where accurate mRNA quantification is essential, such as cancer or disease biomarker discovery, these PNA oligos help minimize the overwhelming presence of globin mRNA, allowing for more precise analysis of gene expression patterns.
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Diagnostic Applications: By improving the quality of RNA sequencing data from blood samples, these oligos contribute to better identification of disease-related gene expression profiles, enhancing the diagnostic potential of blood-based tests.
These oligos are part of broader research efforts to enhance the specificity and reliability of RNA sequencing in human blood samples?. |
