| KKKKKKC, Poly-lysine facilitates the attachment of cells and proteins to solid surfaces in biological applications. Using high pH poly-lysine coating, such as pH 8.5, in borate buffer to enhance the adsorption. Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface. When adsorbed to the culture surface, it increases the number of positively-charged sites available for cell binding. High concentration poly-lysine of 7.5ug/cm² is recommended for the surface area of your culture dish or coverslips. |
