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Protein A Magnetic Beads |
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LTM004 |
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20mg in 10 mM Tris, 0.15 M NaCl, 0.1% BSA,1 mM EDTA, pH7.4, 0.1% NaN3
The magnet is not provided. |
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1-10 mg IgG per ml of beads. |
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~ 1 um diameter |
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Protein A Binding/Washing Buffer (57.7 mM Na2HPO4,42.3 mM NaH2PO4, pH 7.0)
Protein A Elution Buffer (0.2 M Glycine/HCl, pH 2.5)
Protein A Neutralization Buffer (1.0 M Tris-HCl, pH 9.0) |
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1-10 mg protein or 1 mg peptide/mg beads.
Coupling capacity was determined by incubating 30mg beads with human lgG (1 mg/ml in 1 ml PBS) for 60 minutes at room temperature. |
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Product shipped at room temp. Upon receipt, please store at 2-8°C.
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| Stable for at least two years |
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LifeTein Protein A Magnetic Beads are uniform, monodisperse, silica-based superparamagnetic beads covalently conjugated with ultrapure (Purity>97%) recombinant protein A on the surface. The Beads are specifically designed, tested, and quality-controlled for use in immunoprecipitation, and cell sorting when a selected primary antibody is used. The beads are also widely used for quick and efficient one-step purification of IgG antibodies from Serum samples, ascites fluid, plasma, or tissue culture supernatant from several species.
The binding affinity and specificity are summarized below.
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Fast magnetic response,
High binding affinity,
Low non-specific binding,
Compatible with subsequent LC-MS/MS analyses,
Suitable for manual operation or full automation for high throughput analysis
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The beads are simply added to a sample and antibodies will bind to the beads. After washing unbound impurities off, the antibodies can be eluted from the magnetic beads or the bound magnetic beads can be directly used in downstream experiments. The process can be completed manually or fully automated for high-throughput applications.
Download the Protocol HERE. |
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The product is not thoroughly tested and is not intended for human use. For in-vitro and research use only. |
