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RRRRRRRRRKKLFKKILKYL-NH2. R9-BP100 is a cell-penetrating peptide that can be used as a delivery tool for rapid and efficient gene delivery into plant cells. R9-BP100 is made up of nine tandem polyarginine R9 (RRRRRRRRR) and BP100 (KKLFKKILKYL). R9 is a derivative of TAT where all amino acids are substituted for arginine. R9 was known for its effectiveness in both plant and mammalian systems as well as its relatively well-characterized mechanism of action in mammalian cells. BP100 was originally designed and optimized as an antimicrobial peptide to protect against plant pathogens. R9 appeared to be the most effective of the tested CPPs with TAT being 0.77 times as effective as R9, and BP100 or GFP11 alone showing no statistically significant signal (doi: https://doi.org/10.1101/2022.05.03.490515). Image reference: Biomacromolecules 2013, 14, 1, 10-16. |
