T-bet / Tbx21 Rabbit mAb

Product Name
T-bet / Tbx21 Rabbit mAb
Catalog Number
LTA24825
Quantity
100ul
Price
$750 In stock
Description
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.
Alias
TBET; IMD88; T-PET; T-bet; TBLYM
Keywords
30009,sc-11330,sc-11333,sc-21003,T bet,T box 21,T box protein 21,T PET,TBET,TBLYM,TBX21,Transcription factor TBLYM,T-PET,T-bet,T-box 21,T-box transcription factor TBX21,T-box expressed in T cells,T-box protein 21,T-cell-specific T-box transcription factor T-bet,transcription factor TBLYM,Q9UL17,Tbx21,T-Box 21,T-Cell-Specific T-Box Transcription Factor T-Bet,Transcription Factor TBLYM,T-Box Protein 21,T-Box Transcription Factor TBX21,T-Box Expressed In T Cells,T-Bet
Gene Template Species
Human
Gene/Protein ID
30009
SWISS ID
Q9UL17
Antigen Sequence
KGFRENFESM YTSVDTSIPS PPGPNCQFLG GDHYSPLLPN QYPVPSRFYP DLPGQAKDVV PQAYWLGAPR DHSYEAEFRA VSMKPAFLPS APGPTMSYYR GQEVLAPGAG WPVAPQYPPK MGPASWFRPM RTLPMEPGPG GSEGRGPEDQ GPPLVWTEIA PIRPESSDSG LGEGDSKRRR VSPYPSSGDS SSPAGAPSPF DKEAEGQFYN YFPN
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 322-535 of human T-bet/Tbx21(NP_037483.1).
Animal Source
Rabbit
Antibody Type
Affinity Purified Monoclonal IgG
Applications
WB, IP, ELISA
Cross-Reactivity
Human, Mouse, Rat
Molecular Weight
58kDa
Recommended Dilution
WB,1:500 - 1:1000|IP,0.5_g-4_g antibody for 200_g-400_g extracts of whole cells|ELISA,Recommended starting concentration is 1 Āµg/mL. Please optimize the concentration based on your specific assay requirements.
Stability and Storage

Reconstituted antibodies stable at -80C for 12 months, 4C for 1 week. Avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Dissolve the lyophilized antibodies in distilled water. 5-10% glycerol is recommended.

Image
 T-bet / Tbx21 Rabbit mAb

Western blot analysis of various lysates using T-bet / Tbx21 Rabbit mAb (LTA24825) at 1:1000 dilutionĀ incubated overnight at 4_.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LT014) at 1:10000 dilution.|Lysates/proteins: 25 _g per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (LT00020).|Negative control (NC): U-937|Exposure time: 180s.

  • 4 Units in Stock

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