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| T-bet / Tbx21 Rabbit mAb |
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| LTA24825 |
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| 100ul |
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$750
In stock
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| This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. |
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| TBET; IMD88; T-PET; T-bet; TBLYM |
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| 30009,sc-11330,sc-11333,sc-21003,T bet,T box 21,T box protein 21,T PET,TBET,TBLYM,TBX21,Transcription factor TBLYM,T-PET,T-bet,T-box 21,T-box transcription factor TBX21,T-box expressed in T cells,T-box protein 21,T-cell-specific T-box transcription factor T-bet,transcription factor TBLYM,Q9UL17,Tbx21,T-Box 21,T-Cell-Specific T-Box Transcription Factor T-Bet,Transcription Factor TBLYM,T-Box Protein 21,T-Box Transcription Factor TBX21,T-Box Expressed In T Cells,T-Bet |
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| Human |
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| 30009 |
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| Q9UL17 |
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| KGFRENFESM YTSVDTSIPS PPGPNCQFLG GDHYSPLLPN QYPVPSRFYP DLPGQAKDVV PQAYWLGAPR DHSYEAEFRA VSMKPAFLPS APGPTMSYYR GQEVLAPGAG WPVAPQYPPK MGPASWFRPM RTLPMEPGPG GSEGRGPEDQ GPPLVWTEIA PIRPESSDSG LGEGDSKRRR VSPYPSSGDS SSPAGAPSPF DKEAEGQFYN YFPN |
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| Recombinant fusion protein containing a sequence corresponding to amino acids 322-535 of human T-bet/Tbx21(NP_037483.1). |
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| Rabbit |
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| Affinity Purified Monoclonal IgG |
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| WB, IP, ELISA |
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| Human, Mouse, Rat |
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| 58kDa |
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| WB,1:500 - 1:1000|IP,0.5_g-4_g antibody for 200_g-400_g extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
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Reconstituted antibodies stable at -80C for 12 months, 4C for 1 week. Avoid repeated freeze-thaw cycles.
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Shipped at ambient temperature.
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Centrifuge tubes before opening. Dissolve the lyophilized antibodies in distilled water. 5-10% glycerol is recommended.
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| .jpg )
Western blot analysis of various lysates using T-bet / Tbx21 Rabbit mAb (LTA24825) at 1:1000 dilution incubated overnight at 4_.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LT014) at 1:10000 dilution.|Lysates/proteins: 25 _g per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (LT00020).|Negative control (NC): U-937|Exposure time: 180s. |
