Biotin-Ahx-LPETGS

Catalog Number:
5746
Category:
Peptide
Sequence:
Biotin-Ahx-LPETGS-NH2, Biotin–aminohexanoic acid–LPETGS (C-terminal amide)
Modifications:
The majority of Staphylococcus aureus virulence- and colonization-associated surface proteins contain a pentapeptide recognition motif (LPXTG). This motif can be recognized and cleaved by sortase A (SrtA) which is a membrane-bound transpeptidase. The SrtA substrates Biotin–aminohexanoic acid–LPETGS and SELPETGG were used for the interactions between immune cells ‘Labelling Immune Partnerships by SorTagging Intercellular Contacts’ (LIPSTIC). The peptide-receptor interactions enable the direct measurement of dynamic cell–cell interactions. The peptides are flexible tools for use with different receptor–ligand pairs and a range of detectable labels.
Quantity:
5 mg, 100 mg, 1 gram
Purity:
>95%
Molecular Weight:
941.1
Reference:

1. Proximity-dependent labeling identifies dendritic cells that drive the tumor-specific CD4+ T cell response, Science Immunology, 4 Oct 2024, Vol 9, Issue 100, DOI: 10.1126/sciimmunol.adq8843

2. Nakandakari-Higa S, Walker S, Canesso MCC, et al. Universal recording of immune cell interactions in vivo. Nature. 2024 Mar;627(8003):399-406. doi: 10.1038/s41586-024-07134-4.

3. Lee, C.S., Chen, S., Berry, C.T. et al. Fate induction in CD8 CAR T cells through asymmetric cell division. Nature (2024). https://doi.org/10.1038/s41586-024-07862-7

4. Pasqual, G., Chudnovskiy, A., Tas, J. et al. Monitoring T cell–dendritic cell interactions in vivo by intercellular enzymatic labelling. Nature 553, 496–500 (2018). https://doi.org/10.1038/nature25442

 

Reference:
Biotin-Ahx-LPETGS-NH2

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