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LifeTein provides a complete portfolio of antibody services including free peptide antigen design, peptide synthesis, and polyclonal and monoclonal antibody production, development, and purification services. As a leading provider of superior custom antibody development services, LifeTein promotes the understanding of life one protein at a time and offers a wide range of industry-leading platforms and services. LifeTein can customize a discovery and development path to fit your exact needs.
LifeTein has developed the SIMBATM (SIngle Monoclonal B cell Antibody) technology based on single B cell retrieval to identify antigen-specific mAbs. The SIMBATM technology includes single cell RT-PCR with successional cloning and expression in vitro and requires relatively fewer cells. The SIMBATM technology is an attractive approach for retrieval of naive and antigen-experienced antibody repertoires generated in vivo and design of rationale structure-based vaccine.
LifeTein's AdjuBoosterTM adjuvant was developed at our antibody campus core facility. It was proven to increase the immune response in a shorter time than competing products. Our facility uses this innovative new system to generate high-affinity custom antibodies. LifeTein scientists will work with you to generate custom antibodies for your research and development programs.
Featured Service: 🐇 Peptide Affinity Column Purified Polyclonal Antibody Service
We provide the following services:
1. Free Peptide Antigen Design: Stronger antigenicity for the chosen domain.
2. Custom Polyclonal Antibody Services: Peptide affinity column purified antibodies for less background (Choice of Rabbits🐇, Guinea Pigs🐹, Goats🐐,Llama🦙, Rats🐀, or Mice🐁).
3. Custom Mice Monoclonal Antibody Services (Mice 🐁): Guaranteed two positive clones (Mice🐁 Only).
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Reference:
References using synthetic peptides and antibodies from LifeTein. See search results page from the Google Scholar.
LifeTein generated antibodies for Vaccine Analytical Research Development and Vaccine Process Development Merck & Co., Inc., Kenilworth, NJ, USA. The rabbit polyclonal antibodies were generated against the Coxsackievirus A21 (CVA21) capsid proteins: VP1, VP2, VP3, and VP4. All animal work and sera collection were performed at Lifetein, LLC. The sera were affinity-purified by its corresponding peptides.
LifeTein generated Anti-gM and anti-gN antibodies for Vaccine Analytical Research Development and Vaccine Process Development Merck & Co., Inc., Kenilworth, NJ, USA. The rabbit polyclonal anti-gM and anti-gN antibodies were elicited by vaccinating animals with synthesized peptide sequences of gM (1-13aa and 345-372aa) and gN (61-101aa). Animal immunization and sera collection were performed at Lifetein, LLC (Hillsborough, NJ, USA). The sera were affinity-purified by its corresponding peptides.
LifeTein helped synthesized a series of phosphorylated peptides. Then the peptides were used for phospho-specific p-METTL3 (S43) antibody productions. Sun et al. demonstrate that activation of the ERK pathway promotes m6A methylation by phosphorylation of METTL3 and WTAP.
LifeTein made the affinity-purified rabbit polyclonal anti-SclA antibody. The purified streptococcal collagen-like protein A (SclA) antibodies demonstrated that a mutation in Group A Streptococcus carrier strains increase adherence and decrease virulence.
LifeTein helped designed and synthesized a series of phosphorylated peptides. Then the peptides were used for phospho-specific antibody productions. The phospo-specific antibodies by LifeTein were confirmed to react with the epidermal growth factor receptor (EGFR). The Hung's lab showed that AGO2-Y393 phosphorylation mediates EGFR-enhanced cell survival and invasiveness under hypoxia. These findings suggest that modulation of miRNA biogenesis is important for stress response in tumour cells.
... The following peptides were chemically synthesized for antibody production in mice (Lifetein), Elisa verification (Lifetein) and peptide competition assay in immunohistochemistry (IHC)... Supplementary information
George R. Tiller, Potential Roles of Peroxidases in Caenorhabditis Elegans Innate Immunity. 2014
Custom peptide antibodies were provided by LifeTein to study SKPO-1. The SKPO-1 peroxidase domain peptide was synthesized by LifeTein. The polyclonal antibodies were raised in rabbits and then affinity purified to accomplish high specifications and concentrations. The antibodies were successfully used to localize the SKPO-1 protein in hypodermis. It was found that the peroxidase SKPO-1 contributes to the host immune response during infection.
... With respect to having a pAb raised for IHC, I designed a synthetic SKPO-1 peroxidase domain peptide to be chemically synthesized and have a pAb raised in rabbits against said peptide by Lifetein®. This endeavor was successful and is covered in Chapter 3...