Custom Rabbit🐇, Mouse🐇, or Llama🦙 Monoclonal Antibody Service -SIMBATM Technology

Services & Products

Get published in Nature

Order through

Custom Rabbit🐇, Mouse🐇, or Llama🦙 Monoclonal Antibody Services -SIMBATM Technology

Please click here to get a rabbit🐇 (Mouse🐇 or Llama🦙) polyclonal antibody service quote now!

                        Antibody Service Quote

Rabbit monoclonal antibody services using SIMBATM technology (SIngle Monoclonal B cell Antibody) from any immunogens to monoclonal antibodies in four months
  1. Immunize two rabbits (Mouse and Llama are available) with recombinant protein or conjugated peptide/hapten (60 days)
  2. Isolate memory B cells from selected rabbit (14 days)
  3. Sort out antigen-specific memory B cells (MBC) using the characteristics of MBC binding to the antigen-specific surface BCRn by flow cytometry
  4. Amplify antigen-specific MBCs through RT-PCR, vector construction, and high-throughput expression, resulting in the cell culture supernatant of monoclonal antibodies
  5. Validate express recombinant rabbit mAb’s binding to antigen by indirect ELISA
  6. Deliverables: Two positive clones (500ug purified antibodies each); Sequencing results from 15 clones; Full length heavy & light chain gene sequences.

SIMBATM Technology: Based on a single B cell screening technology with a shorter screen time

Strong Affinity: The rabbit monoclonal antibodies are 10-100 times higher affinity than mouse monoclonal antibodies.

High Specificity: The rabbit monoclonal antibodies have improved sensitivity for assays with lower false positive signals.

Improved Stability: The rabbit's IgGs have extra disulfide bonds for improved stability and shelf life.

Broader epitope recognition: The rabbit antibodies recognize small molecules or haptens including lipids, hormones and small drugs. The antibodies can be raised for peptides with modifications, the protein with conformational epitopes or enzyme with cleavage sites.

How is the Rabbit Monoclonal Antibody Made?

Rabbit Monoclonal Antibody Service

Please click here to get a rabbit polyclonal antibody service quote now!

                        Antibody Service Quote


Jia Shen. et al. EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Nature 497, 383–387 (16 May 2013), doi:10.1038/nature12080

LifeTein helped designed and synthesized a series of phosphorylated peptides. Then the peptides were used for phospho-specific antibody productions. The phospo-specific antibodies by LifeTein were confirmed to react with the epidermal growth factor receptor (EGFR). The Hung's lab showed that AGO2-Y393 phosphorylation mediates EGFR-enhanced cell survival and invasiveness under hypoxia. These findings suggest that modulation of miRNA biogenesis is important for stress response in tumour cells.

... The following peptides were chemically synthesized for antibody production in mice (Lifetein Conc.), Elisa verification (LifeteinConc.) and peptide competition assay in immunohistochemistry (IHC)... Supplementary information