Custom Rabbit🐇, Mouse🐇, or Llama🦙 Monoclonal Antibody Services -SIMBA^TM Technology

Please click here to get a rabbit🐇 (Mouse🐇 or Llama🦙) polyclonal antibody service quote now!

                       

Rabbit monoclonal antibody services using SIMBA^TM technology (SIngle Monoclonal B cell Antibody) from any immunogens to monoclonal antibodies in four months

1. Immunize two rabbits (Mouse and Llama are available) with recombinant protein or conjugated peptide/hapten (60 days)
2. Isolate memory B cells from selected rabbit (14 days)
3. Sort out antigen-specific memory B cells (MBC) using the characteristics of MBC binding to the antigen-specific surface BCRn by flow cytometry
4. Amplify antigen-specific MBCs through RT-PCR, vector construction, and high-throughput expression, resulting in the cell culture supernatant of monoclonal antibodies
5. Validate express recombinant rabbit mAb’s binding to antigen by indirect ELISA
6. Deliverables: Two positive clones (500ug purified antibodies each); Sequencing results from 15 clones; Full length heavy & light chain gene sequences.

SIMBA^TM Technology: Based on a single B cell screening technology with a shorter screen time

Strong Affinity: The rabbit monoclonal antibodies are 10-100 times higher affinity than mouse monoclonal antibodies.

High Specificity: The rabbit monoclonal antibodies have improved sensitivity for assays with lower false positive signals.

Improved Stability: The rabbit's IgGs have extra disulfide bonds for improved stability and shelf life.

Broader epitope recognition: The rabbit antibodies recognize small molecules or haptens including lipids, hormones and small drugs. The antibodies can be raised for peptides with modifications, the protein with conformational epitopes or enzyme with cleavage sites.

How is the Rabbit Monoclonal Antibody Made?

Phase	Service	Description	Timeline
Phase I	Antigen Preparation and Immunization	Standard Package: Immunize two rabbits using peptides or recombinatant proteins Bleed titer and measured with ELISA Send the test bleeds to the client Client selects one (or more) rabbit for splenocytes isolation Phase I report	6-9 weeks
Phase IIa	Single B cell Sorting	Antigen specific B cell sorting (by FACS) and culture. High throughput ELISA screening for cultured cell supernatant Selection of ELISA positive clones	2 weeks
Phase IIb	VH/VL amplification & Vector construction	VH/VL gene amplification from selected positive B cells Vector construction & transfer into HEK293T cells ELISA screen for transfected HEK293T cell supernatant	2 weeks *
Phase III	Expression plasmid construction & antibody expression	Subclone VH/VL gene into mammalian expression vector from selected clones Small scale expression and purification of rabbit monoclonal antibodies QC for purified rabbit mAbs Choose 10-15 positive clones for sequencing Send supernatant to the clients for selection Guaranteed two positive purified antibodies (up to 1mg of purified antibodies), IgG VH/VL sequences, and project reports as the final deliverables	3 weeks *

* This timeline is based on the construction of 20 clones and 5 final rabbit antibodies.

Please click here to get a rabbit polyclonal antibody service quote now!

                       

Reference:

Holocentromeres can consist of merely a few megabase-sized satellite arrays Nature CommunicationsNat Commun 14, 3502 (2023). https://doi.org/10.1038/s41467-023-38922-7

LifeTein designed and synthesized a series of peptides: CENH3, MIS12 and NDC80. The peptides were used for immunization of rabbits to generate polyclonal antibodies.

... The peptide synthesis, immunization, and antibody purification were performed by LifeTein...

Dias, Y., Mata-Sucre, Y., Thangavel, G., Costa, L., Báez, M., Houben, A., Marques, A., & Pedrosa-Harand, A. (2024). How diverse a monocentric chromosome can be? Repeatome and centromeric organization of Juncus effusus (Juncaceae). The Plant Journal, 118(6), 1832-1847. https://doi.org/10.1111/tpj.16712

...antibodies in rabbits using the service of Lifetein, LLC (Somerset, NJ...

Jia Shen. et al. EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Nature 497, 383–387 (16 May 2013), doi:10.1038/nature12080

LifeTein helped designed and synthesized a series of phosphorylated peptides. Then the peptides were used for phospho-specific antibody productions. The phospo-specific antibodies by LifeTein were confirmed to react with the epidermal growth factor receptor (EGFR). The Hung's lab showed that AGO2-Y393 phosphorylation mediates EGFR-enhanced cell survival and invasiveness under hypoxia. These findings suggest that modulation of miRNA biogenesis is important for stress response in tumour cells.

... The following peptides were chemically synthesized for antibody production in mice (Lifetein), Elisa verification (Lifetein) and peptide competition assay in immunohistochemistry (IHC)... Supplementary information