LifeTein® offers gene to protein expression service in E.coli. and mammalian cell expression system. Protein expression in bacterial cells is a fast and economical way for high levels of recombinant proteins.
LifeTein's custom bacterial protein expression platform is optimized for low production costs, high scalability, and attractive low pricing for the production of recombinant protein. LifeTein provides the service from the virtual sequence to purified protein in as little as four weeks. Protein production is supplied at flexible scales and formats, including from 100 ml assay scale up to 20 liters of production in suspension culture. You will receive recombinant protein and antibody products that meet your particular specifications and expectation.
Order your E.coli Protein Service at a competitive price! Email us at sales@lifetein.com for a quote.
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It is easy. Just email us a gene sequence or plasmids in any form. We will have the gene designed, optimized, synthesized, and cloned. Or send us the protein sequence and we will send you minigrams of proteins. Proteins are shipped on dry ice. |
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It is flexible. Your choice of getting synthetic genes, plasmids, peptides or proteins. Cloning of any gene of interest into a bacterial expression vector with His-tag or other selected tag (such as GST, His, FLAG or Thioredoxin). If the 6His tag does not give us efficient expression, we will change the 6His tag to GST for free. |
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It is economical. We offer package price from gene synthesis, plasmid preparation, cloning, protein expression to antibody production. |
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It is productive. Optimized refolding methodology is available to solve the protein solubility issues. Enhanced expression vectors are used to increase the protein expression scale and bioactivity. |
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It is large. Need more genes, plasmids, peptides or proteins? We are able to scale up the gene synthesis, plasmid preparation, peptide production or protein expression to hundreds of litres or gram levels. |
1. How long does a mammalian transient protein expression project usually take?
Starting with the mammalian expression plasmid, our service can be completed in 5-9 weeks.
2. What advantages does a mammalian transient protein expression system provide?
Mammalian expression systems can be used when a target protein with posttranslational modifications is needed for functional and structural studies. While peptide synthesis is usually for difficult proteins that are not feasible within any protein expression system. In addition, the eukaryotic system is completely safe. You just need to provide the protein sequence, antibody sequence or hybridoma cells, we will provide the proteins or antibodies.
3. What if my gene does not express well?
We have different expression strains and leader sequences for optimizing expression. Options of refolding methods and tags are used for the best conditions.
Recombinant proteins accumulated intracellularly form inclusion bodies, or insoluble aggregates of misfolded protein lacking biological activity. Proteins have to fold in a correct conformation. In this way, specific sites of the right size, shape, and polarity are created for their ligands or receptors. Conventional methods for refolding of insoluble recombinant proteins include slow dialysis, ion exchange, gel filtration, or dilution into a buffer of near neutral pH.
LifeTein's refolding technology is based on a simple and historically successful protocol. This method reduces protein waste and minimizes unnecessary analysis. The simplified protocol provides reliable, high-yield and high concentration of refolded proteins.
Quotations: Email your protein service inquiry to sales@lifetein.com. We will send you a formal quote within 24 hours.