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Oligonucleotide-peptide conjugates are chimeric molecules composed of a nucleic acid moiety covalently linked to a peptide moiety. It is an important tool for studies of the protein– and peptide–nucleic acid interactions, as well as being promising bioconjugates for nucleic acid delivery and therapy. The technology can be used for cancer immunotherapy techniques, CRISPR/Genome Editing, liquid biopsies, single-cell analysis, nanopore sequencing, nanoparticle drug delivery, and structure-based drug design.
Conjugation of these large and functionalized molecules is a difficult task. In most cases, a direct synthesis of oligonucleotide-peptide conjugates is not possible. However, these conjugation problems are solved by LifeTein's PeptideSyn click chemistry on peptides by protecting or modifying functional groups. Our chemoselective ligation method can form very efficient linkage and reaction between the oligonucleotides and peptides.
Synthetic peptides are valuable tools for laboratory diagnosis. The peptide-based diagnostic tests are to achieve recognition of specific antibodies induced by whole viral proteins by using selected short fragments containing the most potent antigenic determinants. The oligo-peptide conjugate can be used as DNA-directed immobilization for the binding of multiple antigens to a solid support. Moreover, oligopeptide conjugate has recently been shown to improve the therapeutic potential of oligonucleotides in siRNA, cell permeable peptides, and aptamer approaches.
Linear Peptide Oligonucleotide Conjugate
LifeTein offers DNA-peptide conjugate, RNA-peptide conjugate, Morpholino oligomer peptide or phosphorodiamidate Morpholino oligomer (PMO) peptide conjugation for cell screening and in vivo studies. For RNA peptide conjugate, 2′-O-methyl RNA is a recommended analog, which is resistant to degradation by single-stranded ribonucleases. The click chemistry will be used. Both the peptide and oligonucleotide are modified for the bioorthogonal ligation reactions. The phosphorodiamidate Morpholino oligomer (PMO) contains DNA bases attached to a backbone of methylenemorpholine rings linked through phosphorodiamidate groups.
A variety of nanoparticle drug delivery or cellular delivery systems have been developed during the last few years. These include nanoparticles, cell penetrating peptides (CPPs), oligo-aspartic acid, or nuclear localization signal (NLS) peptides to enhance cellular internalization. The oligo-peptide or oligo-CPP concept can be used to overcome the cell membrane barrier and endosomal entrapment. Here are a few CPP examples: NLS:PKKKRKV-CONH2; TAT: RKKRRQRRRPP-CONH2; Arg(9):RRRRRRRRR-CONH2; d-Arg(9): rrrrrrrrr-CONH2; 10HC: HHHHHHHHHHC.
Case Study of Peptide-Oligo Conjugates: The following example is used the identification of single or multiple microorganisms in a sample using FLAG Peptide Labeled Oligonucleotides (PLONs). The PLONs are specifically designed to be complementary to specific nucleic acid sequences on a target microorganism. The hybridized PLON-enzyme coupled FLAG antibody complex is identified by the color reactions. Buy FLAG peptide and antibodies from here.
Multiple Antigenic Peptide Oligonucleotide Conjugate
Cell Permeable Peptide Oligonucleotide Conjugate
Cell-Permeable Peptides (CPPs) or a peptide of choice can be conjugated to an oligonucleotide for drug carrier and delivery.
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