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Oligonucleotide-peptide conjugates represent chimeric molecules comprising a nucleic acid moiety covalently linked to a peptide moiety. This versatile construct serves as a pivotal tool for studying protein– and peptide–nucleic acid interactions and holds significant promise as a bioconjugate for nucleic acid delivery and therapy. The technology finds application in diverse fields such as cancer immunotherapy techniques, CRISPR/Genome Editing, liquid biopsies, single-cell analysis, nanopore sequencing, Lipid Nanoparticles drug delivery, and structure-based drug design.
Conjugating these large and functionalized molecules poses a formidable challenge. In most cases, direct synthesis of oligonucleotide-peptide conjugates is impractical. However, LifeTein addresses these challenges with its PeptideSyn click chemistry on peptides, involving the protection or modification of functional groups. Our chemoselective ligation method facilitates efficient linkage and reaction between oligonucleotides and peptides.
Synthetic peptides are invaluable tools for laboratory diagnosis. Peptide-based diagnostic tests aim to recognize specific antibodies induced by whole viral proteins, utilizing selected short fragments containing the most potent antigenic determinants. The oligo-peptide conjugate proves useful as DNA-directed immobilization for binding multiple antigens to a solid support. Furthermore, oligopeptide conjugates have recently demonstrated enhanced therapeutic potential for oligonucleotides in siRNA, cell permeable peptides, and aptamer approaches.
Traceless Protein-CPP Conjugation
LifeTein introduces a versatile strategy for the bioreversible modification of proteins for cellular delivery, incorporating efficient cell-penetrating peptides (CPPs). Our strategy relies on a tricomponent molecule that includes a diazo moiety for the chemoselective esterification of carboxyl groups on the target protein. The linker and CPP will be removed by a self-immolative carbonate group, facilitating esterase-mediated cleavage. This innovative approach offers a traceless modification, ensuring minimal interference and optimal cellular delivery of the protein-CPP conjugate.
Linear Peptide-Oligonucleotide Conjugate
LifeTein offers a range of conjugation services, including DNA-peptide conjugate, Peptide Nucleic Acids (PNAs)-peptide conjugates, RNA-peptide conjugates, Morpholino oligomer peptides, and phosphorodiamidate Morpholino oligomer (PMO) peptide conjugation for cell screening and in vivo studies. For RNA peptide conjugates, the recommended analog is 2′-O-methyl RNA, resistant to degradation by single-stranded ribonucleases. Click chemistry is employed, modifying both the peptide and oligonucleotide for bioorthogonal ligation reactions.
The development of various nanoparticle drug delivery and cellular delivery systems in recent years has included Clickable Lipids, cell-penetrating peptides (CPPs), oligo-aspartic acid, or nuclear localization signal (NLS) peptides to enhance cellular internalization. The oligo-peptide or oligo-CPP concept proves effective in overcoming cell membrane barriers and endosomal entrapment. Examples of CPPs include NLS:PKKKRKV-CONH2; TAT: RKKRRQRRRPP-CONH2; Arg(9):RRRRRRRRR-CONH2; d-Arg(9): rrrrrrrrr-CONH2; 10HC: HHHHHHHHHHC.
Case Study of Peptide-Oligo Conjugates: Illustrating the utility of peptide-oligo conjugates, this case study involves the identification of single or multiple microorganisms in a sample using FLAG Peptide Labeled Oligonucleotides (PLONs). Specifically designed PLONs complement specific nucleic acid sequences on a target microorganism. The hybridized PLON-enzyme coupled FLAG antibody complex is identified through color reactions. Obtain FLAG peptide and antibodies here.
Multiple Antigenic Peptide Oligonucleotide Conjugate
Cell Permeable Peptide Oligonucleotide Conjugate
Cell-Permeable Peptides (CPPs) or a peptide of choice can be conjugated to an oligonucleotide for drug carrier and delivery.
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