Monthly Archives: January 2018

How HIV-1 integrase contributes to virion morphogenesis?

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It was found that the HIV-1 Integrase binds the Viral RNA genome and is essential during Virion Morphogenesis. The L50 Peptide: Cyclo-(-Arg-Val-Arg-Thr-Arg-Gly-LysArg-Arg-Ile-Arg-Arg-DPro-Pro-) was synthesized by LifeTein and used for the inhibition assays. The L50 is a Tat-derived peptide sequence, which selectively engages both the loop and 3-nt bulge
but not the double-stranded stem of the transactivation response. The TAT-derived peptide is a well-defined cell penetration peptide.

HIV-1 Integrase Binds the Viral RNA genome

Cell Penetration Peptides

Cell Penetration Peptides

Monitoring T cell–dendritic cell interactions in vivo using labeled peptides

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The SrtA substrates Biotin–aminohexanoic acid–LPETGS and SELPETGG were used to interact with immune cells ‘Labelling Immune Partnerships by SorTagging Intercellular Contacts’ (LIPSTIC). The peptide-receptor interactions enable the direct measurement of dynamic cell-cell interactions. The peptides are flexible tools with different receptor-ligand pairs and a range of detectable labels.

peptide-receptor interaction

Schematic representation of the LIPSTIC approach

 

Nature, Monitoring T cell–dendritic cell interactions in vivo by intercellular enzymatic labeling

Peptide Biotin–aminohexanoic acid–LPETGS, SELPETGG was purchased from LifeTein.