To improve the design of immunosensors, fluorophores were conjugated on to the single chain Fv (scFv) fragment of a recombinant anti-BGP antibody to form Quenchbodies (Q-bodies), in which Trp residues of scFv internally quench their fluorescence.  Unlabeled and biotinylated BGP peptides made by LifeTein were used in ELISA and FRET assays as the ligand to evaluate the quality of Qbodies, and to demonstrate the antigen-dependent fluorescence of Qbodies.

Making Simpler & Better Biosensors

ACS Sensors, Article ASAP. DOI: 10.1021/acssensors.5b00089.  Q-Bodies from Recombinant Single-Chain Fv Fragment with Better Yield and Expanded Palette of Fluorophores.