Dimethyl sulfoxide (DMSO) is a polar, aprotic organic solvent widely utilized in cell culture, especially as a cryoprotectant to prevent ice crystal formation during freezing. Its unique membrane-penetrating and water displacement properties help safeguard cells from death caused by freezing, typically at concentrations around 10% when combined with saline or serum albumin (Eppendorf Handling Solutions).
However, DMSO's application extends beyond cryopreservation, especially in dissolving hydrophobic peptides, which can be challenging to solubilize in aqueous solutions due to their inherent properties. The introduction of DMSO can enhance cell permeability, making it a valuable tool for delivering such peptides into cells. Yet, caution is necessary as DMSO concentrations exceeding 0.5% might induce cytotoxic effects, jeopardizing cell viability. While most cell lines can endure up to 0.5% DMSO with minimal cytotoxicity, primary cells often require even lower concentrations, not exceeding 0.1%, to avoid adverse effects (LifeTein).
To safely introduce peptides dissolved in DMSO into cell culture:
- Dissolve peptides in a minimal volume of DMSO, aiming for a concentration that allows for further dilution in the cell culture medium.
- Dilute the DMSO-peptide solution gradually in an aqueous buffer solution, such as PBS, to achieve the desired final peptide concentration in the cell culture. Vigilance is needed to prevent the solution from becoming turbid, indicating solubility limits have been reached. Sonication might aid in dissolving the peptides further (LifeTein).
- Final concentration adjustment is crucial, ensuring that DMSO does not exceed safe levels for the cell type in culture. Keeping the final DMSO concentration around 0.1% is advisable to ensure compatibility with almost all cell lines. Up to 0.5% can be employed for applications necessitating higher DMSO concentrations, provided cell viability is not compromised (LifeTein).
By adhering to these guidelines, researchers can leverage DMSO's solubilizing properties for hydrophobic peptides while minimizing potential cytotoxic effects on cell cultures. Including controls treated with DMSO alone in experiments is essential to discern any DMSO-related impacts from those of the peptide of interest. This balanced approach allows the exploration of peptide functions in cellular contexts without compromising cell health and experiment integrity.