How to Coat the Cell Culture Plate with RGD Peptides

How to Coat the Cell Culture Plate with RGD Peptides

RGD products: 

• biotin-Ahx-PPPPRGDRGDRGD-NH2
• Cyclo RGD peptide, CRGDKGPDC-NH2

More information about the RGD peptides. 

Usage Instructions:

Note: These recommendations should be used as guidelines to determine the optimal coating conditions for your culture system. All operations should be performed in a laminar flow hood to maintain sterility. Two procedures are provided: Procedure A and Procedure B.

Procedure A:

1. Remove the cap and add 5 ml of serum-free medium or PBS to the bottle.

2. Replace the cap and vigorously vortex the contents. Make sure that the RGD peptide is completely dissolved. The solution will remain slightly cloudy.

3. Transfer the desired volume of the solution from the bottle to a dilution vessel. Dilute it to the desired concentration using a serum-free medium or PBS. A typical working concentration may range from 0.1 to 10 μg/ml.

4. Add the appropriate amount of diluted material to the culture surface.

5. Incubate at room temperature or 37°C, covered, for 1-2 hours.

6. After incubation, remove any remaining material.

7. Carefully rinse the plates with dH2O, avoiding scratching the bottom surface of the plates.

8. The plates are now ready for use. They may be stored at 2-10°C, air-dried, as long as sterility is maintained.

9. Store any remaining solubilized RGD peptide at 2-10°C.

Note: To achieve optimal cell binding, the cell attachment solution should include divalent cations (Calcium, Magnesium, or Manganese).

Procedure B:

1. Remove the cap and add 5 ml of sterile 70% ethanol.

2. Replace the cap and vortex the contents. Make sure that the RGD peptide is completely dissolved.

3. Transfer the desired volume of the solution from the bottle to a dilution vessel. Dilute it to the desired concentration using 70% ethanol. Concentrations from 0.1 to 10 μg/ml should be tested.

4. Add the appropriate amount of diluted material to the culture surface.

5. Leave the coated container uncovered in a laminar flow hood until the wells are dry.

6. Carefully rinse the plates with dH2O, avoiding scratching the bottom surface of the plates.

7. The plates are now ready for use.

8. Store any remaining solubilized RGD peptide at 2-10°C.