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| Biotinylated Human ANGPTL3/Angiopoietin-like 3 Protein (LTP11036) |
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| LTP11036 |
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| 100ug |
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$789 In stock |
| ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C?terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake. |
| Recombinant Biotinylated Human ANGPTL3/Angiopoietin-like 3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ser17-Lys219. |
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| ANGPTL3/Angiopoietin-like 3 |
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| Human |
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| Q9Y5C1 |
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| Ser17-Lys219 |
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| The protein has a predicted MW of 26.6 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-ANGPTL3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ANGPTL3, His Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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