|
| Biotinylated Human B7-2/CD86 Protein (LTP11026) |
|
| LTP11026 |
|
| 100ug |
|
|
$789 In stock |
| B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. |
| Recombinant Biotinylated Human B7-2/CD86 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu26-Pro247. |
|
| B7-2/CD86 |
|
| Human |
|
| P42081-1 |
|
| Leu26-Pro247 |
|
| The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on the Tris-Bis PAGE result. |
|
| Immobilized Human CTLA-4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human B7-2, His Tag with the EC50 of 3.8ug/ml determined by ELISA. See testing image for detail. |
|
| C-His-Avi |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
