| The peptide sequence DYKDDDDKGGGGSYPYDVPDYA appears to be a modified version of the DYKDDDDK tag and HA tag, which is commonly referred to as the FLAG tag and HA tag. This peptide is often used in molecular biology and biochemistry for various research applications. Here's a breakdown of the components in this peptide:
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DYKDDDDK: This is the core FLAG tag sequence. It consists of eight amino acids (Aspartic acid, Tyrosine, Lysine, Aspartic acid, Aspartic acid, Aspartic acid, Aspartic acid, Lysine) and is typically recognized by antibodies specifically designed to target the FLAG tag. Researchers often use this tag to label proteins of interest, making them easier to detect and purify in experiments.
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GGGGS: This sequence is often added to the C-terminus of the FLAG tag for additional flexibility and spacing. It consists of five glycine (G) residues followed by a serine (S). The glycine residues provide a flexible linker, which can help prevent interference between the FLAG tag and the protein of interest.
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YPYDVPDYA: An HA-tag is derived from the human influenza hemagglutinin (HA) molecule corresponding to amino acids 98-106.
In summary, the peptide DYKDDDDKGGGGSYPYDVPDYA is a modified version of the FLAG-HA tag with a GGGGS linker, which is commonly used for protein labeling and detection in molecular biology research. |
