Peptide Synthesis: Handling and Storage of Synthetic Peptides

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Frequently Asked Questions (FAQ)

References using synthetic peptides and antibodies from LifeTein. Full Publication List of 2017.

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How to dissolve peptides containing free cysteines?

Peptides containing a single free cysteine will be oxidized at pH>7 to form dimers. This oxidation can be reverted. Peptides containing two or more thiol moieties may yield a mixture of products upon oxidation. A pH 7.5-8 is the best condition for the disulfide bond formation. Hence, peptides containing free cysteines are best dissolved in degassed solvents, e.g. buffers pH<7, diluted acetic acid, 0.1% trifluoroacetic acid in aqueous acetonitrile. DMSO should be avoided, especially with peptide trifluoroacetate.